E07H0106 Porcine Serotonin/5 Hydroxytryptamine ELISA kit
Porcine Serotonin/5 Hydroxytryptamine ELISA kit can be used to identify samples from the porcine species. Serotonin/5 Hydroxytryptamine can also be called 5HT; Serotonin; N-Methyl-Gamma.
Specifications of Porcine Serotonin/5 Hydroxytryptamine ELISA kit
Product Information | |
Cat. No. | E07H0106 |
Product Name | Porcine Serotonin/5 Hydroxytryptamine ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 25 ng/mL | 1 vial |
STANDARD C (0.5mL) | 50 ng/mL | 1 vial |
STANDARD D (0.5mL) | 100 ng/mL | 1 vial |
STANDARD E (0.5mL) | 250 ng/mL | 1 vial |
STANDARD F (0.5mL) | 500 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
5 HT ELISA kit uses an anti-5 HT antibody and a 5 HT-HRP conjugate in a competitive enzyme immunoassay method. 5 HT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-5 HT antibody binding site between 5 HT from samples and 5 HT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of 5 HT. Since the number of sites is limited, as more sites are occupied by 5 HT from the sample, fewer sites are left to bind 5 HT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 5 HT concentration in each sample is interpolated from this standard curve. |
Quality Control on Porcine Serotonin/5 Hydroxytryptamine ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between 5 HT and analogues was observed. | |
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Summary of the Assay Procedure for Porcine Serotonin/5 Hydroxytryptamine ELISA kit
Citations of Porcine Serotonin/5 Hydroxytryptamine ELISA kit
E07H0106 has been referenced in the below publications：
Positive effects of a Clostridium butyricum-based compound probiotic on growth performance, immune responses, intestinal morphology, hypothalamic neurotransmitters, and colonic microbiota in weaned piglets.
